Effects of heme oxygenase-1 on proteins related to apoptosis in INS-1 cells exposed to intermittent high glucose.
- Author:
Yi-guang CHEN
1
;
Yao-ming XUE
;
Mei-ping GUAN
;
Bo ZHU
;
Jian-ping SHA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Caspase 3; metabolism; Cell Line; Glucose; administration & dosage; adverse effects; Heme Oxygenase (Decyclizing); metabolism; Islets of Langerhans; cytology; drug effects; Proto-Oncogene Proteins c-bcl-2; metabolism; Rats; bcl-2-Associated X Protein; metabolism
- From: Journal of Southern Medical University 2010;30(10):2330-2332
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of heme oxygenase-1(HO-1) on proteins related to apoptosis in INS-1 cells with exposure to intermittent high glucose.
METHODSINS-1 cells cultured in vitro were divided into control group, persistent high glucose group (PHG), intermittent high glucose group (IHG), CoPP + intermittent high glucose group (CoPP+IHG), and ZnPP+ intermittent high glucose group (ZnPP+IHG). After 72 h of treatment with the corresponding protocols, the cells were examined for expressions of HO-1 protein by Western blotting and for expressions of Bax and Bcl-2 by immunocytochemistry.
RESULTSIn comparison with the control group, the cells in both PHG group and IHG group showed significantly increased expressions of HO-1 (P<0.01) and decreased Bcl-2/Bax ratios (P<0.05). The cells in CoPP+ IHG group exhibited a greater HO-1 protein expression but a lower Bcl-2/Bax ratio than those in IHG group (P<0.05) The ZnPP+IHG group demonstrated opposite changes in terms of HO-1, Bax and Bcl-2 expressions compared with the CoPP+IHG group.
CONCLUSIONIntermittent high glucose can lower Bcl-2/Bax ratio in INS-1 cells, and HO-1 may protect INS-1 cells against apoptosis possibly by up-regulating the Bcl-2/Bax ratio.
