Effects of AMPK on the transcriptional activity of FOXO1 and ubiquitin ligase MuRF1 expression in rat cardiomyocytes.
- Author:
Bao-lin CHEN
1
;
Zhao-jun XIONG
;
Cheng-xi ZHANG
;
Yue-dong MA
;
Rong-sen MENG
;
Guang-qin CHEN
;
Chen LIU
;
Yu-gang DONG
Author Information
- Publication Type:Journal Article
- MeSH: AMP-Activated Protein Kinases; metabolism; Aminoimidazole Carboxamide; analogs & derivatives; pharmacology; Animals; Cells, Cultured; Forkhead Transcription Factors; metabolism; Muscle Proteins; metabolism; Myocytes, Cardiac; metabolism; Nerve Tissue Proteins; metabolism; Rats; Rats, Sprague-Dawley; Ribonucleotides; pharmacology; Tripartite Motif Proteins; Ubiquitin-Protein Ligases; metabolism
- From: Journal of Southern Medical University 2010;30(11):2419-2422
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of AICAR on the activity of transcription factor FOXO1 and expression of ubiquitin ligase MuRF1 in rat cardiomyocytes, and explore the possible role of AMP-activated protein kinase (AMPK) in proteolysis pathways.
METHODSIn vitro cultured neonatal rat cardiac myocytes were treated with AICAR, and Western blotting was used to detect the phosphorylation of FOXO1 and expression of MuRF1 in the cells.
RESULTSAICAR activated AMPK in rat cardiac myocytes. Activated AMPK significantly inhibited the phosphorylation of FOXO1 and increased MuRF1 protein expression.
CONCLUSIONAMPK may regulate proteolysis by activating FOXO1 transcription factor and up-regulating MuRF1 expression.
