Expression of CD80 and CD86 on dendritic cells of patients with immune related pancytopenia and its clinical significance.
- Author:
Guang-shuai TENG
1
;
Rong FU
;
Hui LIU
;
Hong-lei WANG
;
Yi-hao WANG
;
Er-bao RUAN
;
Wen QU
;
Yong LIANG
;
Guo-jin WANG
;
Xiao-ming WANG
;
Hong LIU
;
Yu-hong WU
;
Jia SONG
;
Hua-quan WANG
;
Li-min XING
;
Jing GUAN
;
Jun WANG
;
Li-juan LI
;
Zong-hong SHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Autoimmune Diseases; complications; B7-1 Antigen; metabolism; B7-2 Antigen; metabolism; Case-Control Studies; Child; Child, Preschool; Dendritic Cells; metabolism; Female; Flow Cytometry; Humans; Male; Middle Aged; Pancytopenia; blood; etiology; pathology; Young Adult
- From: Chinese Journal of Hematology 2012;33(10):865-868
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the function of dendritic cells (DC) of patients with immune related pancytopenia (IRP) and explore the role of DC in IRP.
METHODSThe expression of CD80 and CD86 on myeloid DC (mDC, Lin-HLA-DR(+) CD11c(+) cells) and plasmacytoid DC (pDC, Lin-HLA-DR(+) CD123(+) cells) of 65 IRP (37 untreated and 28 remitted) patients and 17 healthy controls were analyzed by flow cytometry.
RESULTSThe expression of CD86 on pDC was (82.47 ± 13.17)% in untreated group and (60.08 ± 14.29)% in remission group, which were significantly higher than that of controls (47.95 ± 18.59)% (P < 0.05), while the expression in untreated group was higher than that of remission group (P < 0.05). The expression of CD80 on pDC was (6.31 ± 4.49)% in untreated group, which was significantly higher than that of remitted patients (3.09 ± 2.93)% and controls (2.33 ± 2.25)% (P < 0.05). The expression of CD86 on mDC was (97.06 ± 4.82)% in untreated group and (91.35 ± 12.20)% in control group, while the expression in untreated group was higher than that of control group (P < 0.05). The expression of CD80 on mDC was (6.20 ± 5.44)% in untreated group and (3.97 ± 3.24)% in remission group, which were significantly higher than that of controls (1.86 ± 1.73)% (P < 0.05). The expression of CD86 on pDC was negatively correlated to Th1/Th2 (r = -0.733, P < 0.05), it was positively correlated to the antibody on membrane of BMMNC (r = 0.283, P < 0.05) and the quantity of CD5(+)B cells (r = 0.436, P < 0.05), while it was negatively correlated to the level of hemoglobin, platelets and white blood cells (r = -0.539, P < 0.05; r = -0.519, P < 0.05; r = -0.567, P < 0.05, respectively). The expression of CD80 on pDC was negatively correlated to the level of hemoglobin and platelets (r = -0.431, P < 0.05; r = -0.464, P < 0.05).
CONCLUSIONThe function of pDC in PB of IRP were strengthened, which was relevant to the immunopathogenesis of IRP.
