Detection of the expression of NK ligands in acute leukemia cell lines by real-time PCR.
- Author:
Yuan LUO
1
;
Lian-ning DUAN
;
Cheng-rong LU
;
Qing CAI
;
Zhe WANG
;
Rong-feng BAO
;
Pei-de XIANG
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Antigens, CD; genetics; metabolism; CD48 Antigen; Cell Line, Tumor; GPI-Linked Proteins; genetics; metabolism; HL-60 Cells; Humans; Intercellular Signaling Peptides and Proteins; genetics; metabolism; Leukemia; genetics; metabolism; Leukemia, Myeloid, Acute; genetics; metabolism; Ligands; Membrane Proteins; genetics; metabolism; Precursor Cell Lymphoblastic Leukemia-Lymphoma; genetics; metabolism; Real-Time Polymerase Chain Reaction; Receptors, TNF-Related Apoptosis-Inducing Ligand; genetics; metabolism; Receptors, Virus; genetics; metabolism
- From: Chinese Journal of Hematology 2013;34(1):26-29
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression profile of NK ligands in acute leukemia cell lines and investigate the differential expression pattern between acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML).
METHODSUsing quantitative real-time PCR, 23 NK ligands (MICA, MICB, ULBP-1, ULBP-2, ULBP-3, ULBP-4, HLA-E, HLA-G, CD48, NBTA, HLA-F, LLT-1, PVR, Nectin2, CD72, CD80, ICAM-1, LFA-3, CRACC, Fas, DR4, DR5, TNFR1) were detected in 6 acute leukemia cell lines, including 3 ALL cell lines (CEM, Jurkat T, Reh) and 3 AML cell lines (HL-60, KG-1a, NB4), respectively. Independent-samples t test analysis was performed to determine statistical significance.
RESULTSUsing β-actin as reference gene, the relative expression results showed that the expression of 4 NK ligands between ALL and AML is significantly different. Specifically, the level of ULBP-2 is higher in ALL (CEM: 1, Jurkat T: 0.617, Reh: 0.246) than that in AML (HL-60: 0.000, KG-1a: 0.003, NB4: 0.000)(P = 0.047). However, the expressions of CD48, PVR(PVR-1, PVR-2) and DR4 is higher in AML (HL-60: 13.987, 4.403, 10.334, 8.711; KG-1a: 5.387, 2.900, 7.315, 4.512; NB4: 7.763, 3.248, 7.049, 6.127) than that in ALL (CEM: 1, 1, 1, 1; Jurkat T: 2.035, 1.553, 3.888, 0.449; Reh: 1.559, 0.000, 0.000, 1.304) (P = 0.044, 0.014, 0.014, 0.011). And there're no significant differences between the rest 19 NK ligands.
CONCLUSIONSULBP-2, CD48, PVR and DR4 might play an important role in the distinct mechanisms in leukemogenesis between ALL and AML and could be potential targets for diagnosis and treatment.
