Impact of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2.
- Author:
Li-quan ZHOU
1
;
Hui ZHANG
;
Xue-song GAO
;
Jian WANG
;
Rui-xia LIANG
;
Bao-fa HONG
;
Jian-guang ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Down-Regulation; Gene Expression; Humans; Male; Phosphoric Diester Hydrolases; biosynthesis; genetics; Prostatic Neoplasms; genetics; metabolism; pathology; Pyrophosphatases; biosynthesis; genetics; RNA Interference; RNA, Messenger; genetics; Transfection
- From: National Journal of Andrology 2005;11(4):256-260
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2.
METHODSRecombinant plasmids of expressing short hairpin RNA targeting PC-1 mRNA were constructed using DNA recombinant technology and transfected into C4-2 cells via liposome. The positive cell clones were selected by G418. The expression of PC-1 gene was analyzed by RT-PCR and Western blotting technology. MTT and soft agar cloning formation were applied to observe the changes of the growth rate and independent anchor ability of C4-2 cells.
RESULTSPC-1 RNA interference severely affected the expression of PC-1 gene and reduced the growth and colony formation ability of C4-2 cells.
CONCLUSIONRNA interference-mediated PC-1 gene knockdown can decrease the growth and cloning formation ability of C4-2 cells.
