Effects of pentachlorophenol on rat sertoli cells.
- Author:
Shu-zhen YANG
1
;
Xiao-dong HAN
;
Wei CHEN
;
Da-qiang YIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Survival; drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Male; Pentachlorophenol; toxicity; Rats; Rats, Sprague-Dawley; Sertoli Cells; drug effects; physiology
- From: National Journal of Andrology 2005;11(4):261-268
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the toxic effects of pentachlorophenol (PCP) on cultured rat Sertoli cells.
METHODSThe viability of Sertoli cells was detected and morphological examination was performed, followed by flow cytometric assay to evaluate the toxic effect of PCP on rat Sertoli cells.
RESULTSMTT assay showed that PCP induced a concentration- and time-dependent decrease in Sertoli cell viability. Flow cytometric assay revealed that the number of dead Sertoli cells grew along with increased exposure to PCP.
CONCLUSIONPCP, with obvious cytotoxic effects, can cause necrosis of Sertoli cells in vitro.
