Feasibility of muscle-derived cell autotransplantation as a treatment for post-prostatectomy urinary incontinence.
- Author:
Qi-sheng YAO
1
;
Zhang-qun YE
;
Cong-bo CHEN
;
Xiao-kang WANG
;
Wei-min WANG
;
Li-xin CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Survival; Cell Transplantation; Female; Muscle, Skeletal; cytology; Postoperative Complications; therapy; Prostatectomy; Rats; Rats, Sprague-Dawley; Transplantation, Autologous; Urethra; cytology; Urinary Bladder; cytology; Urinary Incontinence; etiology; therapy; beta-Galactosidase; genetics
- From: National Journal of Andrology 2005;11(4):272-277
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the feasibility of muscle-derived cell autotransplantation in the treatment of post-prostatectomy urinary incontinence.
METHODSSkeletal muscle-derived cells (MDC) were isolated and purified by replate technique from 6 female SD rats, and then transduced with adenovirus carrying Lac-Z gene. About 5 x 10(6) of the transduced cells were injected autologously into the bladder neck of the animals. Tissues were harvested after 5 and 15 days for histological examination and X-gal staining.
RESULTSAt 5 and 15 days after the autologous MDC transplantation, histological examination revealed no apparent sign of inflammation and inflammatory cell invasion, and X-gal staining showed a large number of cells dyed blue, indicating the survival of the autologous cells.
CONCLUSIONAutotransplanted MDCs can survive permanently. Autologous muscle stem cell injection can be an effective treatment for post-prostatectomy urinary incontinence.
