Comparison of three methods for evaluating acrosome reaction in human spermatozoa.
- Author:
Yun ZHANG
1
;
Qi-Xuan XIE
;
Shan-Pei PAN
;
Chun-Xue ZHANG
;
Luan-Juan XIAO
;
Ya-Lin PENG
Author Information
- Publication Type:Journal Article
- MeSH: Acid Phosphatase; Acrosome Reaction; drug effects; Cells, Cultured; Chlortetracycline; Humans; Male; Progesterone; pharmacology; Rosaniline Dyes; Spermatozoa; cytology; Staining and Labeling; methods
- From: National Journal of Andrology 2005;11(6):419-425
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo find a convenient and exact method for evaluating acrosome reaction in human spermatozoa.
METHODSThe semen of the normal male was mixed and then divided into 6 groups. Coomassie brilliant blue (CBB) staining, chlortetracycline (CTC) fluorescence staining and acid phosphatase (ACP) detection were used for morphological observation and data analysis of the acrosome status of the human sperm treated with or without progesterone.
RESULTSThere were obvious morphological differences between the acrosome-reaction and acrosome-intact spermatozoa in CBB staining and CTC fluorescence staining, and significant differences were observed between the experimental and control spermatozoa by the three methods (P < 0.05).
CONCLUSIONAll the three methods can be used to assess acrosome reaction in human spermatozoa, but Coomassie brilliant blue (CBB) staining is much more convenient and stable.
