Effects of hypothermia on antioxidant capacity of rat testes after testicular torsion.
- Author:
Hai-Tao CHEN
1
;
Xin-Min ZHENG
;
Zhi-Wei YANG
;
Hang ZHENG
;
Shi-Wen LI
;
Li-Quan HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antioxidants; metabolism; Hypothermia, Induced; Lipid Peroxidation; physiology; Male; Malondialdehyde; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Spermatic Cord Torsion; pathology; physiopathology; Testis; pathology
- From: National Journal of Andrology 2005;11(6):426-430
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of hypothermia on the antioxidant capacity of rat testes after testicular torsion.
METHODSTwenty-four healthy pubertal male Sprague-Dawley rats were randomly divided into three groups of equal number: Group A (torsion) , Group B (torsion + hypothermia) and Group C (control). The animals were submitted to unilateral 720 degrees testicular torsion, and underwent detorsion two hours later. Fourteen days later, the total antioxidant capacity (T-AOC) and the level of malonic diethylaldehyde(MDA) were detected with spectrophotometer and histological changes were observed by light microscope.
RESULTSThe T-AOC of Group B was significantly greater than that of Group A (P < 0.01), but less than that of Group C (P < 0.01). The MDA level of Group B was lower than that of Group A (P < 0.01), but higher than that of Group C (P < 0.05).
CONCLUSIONHypothermia can restrain the production of oxygen free radicals following testicular torsion/detorsion in rats, which in turn can inhibit lipid peroxidation and increase the survivability of the torsional testis.
