Intracytoplasmic sperm injection using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration.
- Author:
Xiao-ming TENG
1
;
Li-ping ZHU
;
Kun-ming LI
;
Yu WANG
;
Hong-xia YAO
;
Jing-yu SHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Biopsy, Fine-Needle; Cryopreservation; Female; Humans; Male; Middle Aged; Oligospermia; pathology; therapy; Pregnancy; Semen Preservation; Sperm Injections, Intracytoplasmic; methods; Testis; pathology; Treatment Outcome
- From: National Journal of Andrology 2005;11(7):517-519
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of intracytoplasmic sperm injection (ICSI) using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration (TEFNA) in patients with non-obstructive azoospermia.
METHODSSixty-two patients underwent TEFNA. Mature testicular spermatozoa were found in 35 cases of patients and the testicular tissues were cryopreserved for later ICSI. Ovarian stimulation included a long protocol of GnRHa/FSH/hCG. Oocyte retrieval was performed under transvaginal ultrasound guidance, and ICSI conducted with cryopreserved-thawed testicular spermatozoa.
RESULTSA total of 35 couples underwent 35 ICSI cycles using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration. The clinical pregnancy rate was 37.14% (13/35).
CONCLUSIONICSI using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration is a main and effective method in the treatment of non-obstructive azoospermia, which can avoid further testicular fine needle aspiration.
