Topical application of epidermal growth factor accelerates wound healing by myofibroblast proliferation and collagen synthesis in rat.
- Author:
Young Bae KWON
1
;
Hyun Woo KIM
;
Dae Hyun ROH
;
Seo Yeon YOON
;
Rong Min BAEK
;
Jeum Yong KIM
;
Hae Yong KWEON
;
Kwang Gill LEE
;
Young Hwan PARK
;
Jang Hern LEE
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: alpha-smooth muscle actin; proliferating cell nuclear antigen; prolyl 4-hydroxylase; recombinant human epidermal growth factor; wound healing
- MeSH: Actins/genetics/metabolism; Administration, Topical; Animals; Cell Proliferation/drug effects; Collagen/*biosynthesis; Epidermal Growth Factor/*administration&dosage/*pharmacology; Gene Expression Regulation; Male; Myoblasts, Skeletal/*drug effects; Proliferating Cell Nuclear Antigen/genetics/metabolism; Rats; Rats, Sprague-Dawley; Wound Healing/*drug effects
- From:Journal of Veterinary Science 2006;7(2):105-109
- CountryRepublic of Korea
- Language:English
- Abstract: Recombinant human epidermal growth factor (rhEGF) stimulates the proliferation and migration of epithelial cells in human cell culture systems and animal models of partial-thickness skin wounds. This study investigated the effect of a topical rhEGF ointment on the rate of wound healing and skin re-epithelialization in a rat full thickness wound model, and verified whether or not the rhEGF treatment affected both myofibroblast proliferation and collagen synthesis in the dermis. When rhEGF (10 microgram/g ointment) was applied topically twice a day for 14 days, there was significantly enhanced wound closure from the 5th to the 12th day compared with the control (ointment base treatment) group. A histological examination at the postoperative 7th day revealed that the rhEGF treatment increased the number of proliferating nuclear antigen immunoreactive cells in the epidermis layer. In addition, the immunoreactive area of alpha-smooth muscle actin and the expression of prolyl 4-hydroxylase were significantly higher than those of the control group. Overall, a topical treatment of rhEGF ointment promotes wound healing by increasing the rate of epidermal proliferation and accelerating the level of wound contraction related to myofibroblast proliferation and collagen deposition.
