Effects of Astaxanthin on the damage of osteoblast induced by H2O2.
- Author:
Ling-peng PEI
1
;
Fu-hui DONG
;
Bo-di HUI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antioxidants; chemistry; pharmacology; Cell Line; Hydrogen Peroxide; metabolism; Lipid Peroxidation; drug effects; Membrane Fluidity; drug effects; Mice; Osteoblasts; drug effects; metabolism; Oxidative Stress; drug effects; Reactive Oxygen Species; metabolism; Superoxide Dismutase; metabolism; Xanthophylls; chemistry; pharmacology
- From: China Journal of Orthopaedics and Traumatology 2008;21(3):187-189
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Astaxanthin on enhancing the function of anti-oxidative damage in osteoblast.
METHODSMC3T3-E1 osteoblasts were randomly divided into five groups, including control group, model group, Astaxanthin group [low-dose (1 x 10(-7) mol/L), middle-dose (1 x 10(-6) mol/L), high-dose (1 x 10(-5) mol/L)], in which the activity of cells, activity of superoxide dismutase (SOD), the content of reactive oxygen species (ROS), lipid oxygen (LPO) and membrane fluidity were tested and compared.
RESULTSCompared with Astaxanthin groups, the activity of cells, SOD activity and membrane fluidity in the model group were significantly decreased (P < 0.01). However, the contents of ROS and LPO were significantly raised (P < 0.01).
CONCLUSIONH2O2 can cause oxidative damage of MC3T3-E1 osteoblasts, but Astaxanthin can prevent or decrease its influence.
