Identification of Daturae flos and its adulterants based on DNA barcoding technique.
- Author:
Jian-ping HAN
1
;
Mei-ni LI
;
Kun LUO
;
Mei-zi LIU
;
Xiao-chen CHEN
;
Shi-lin CHEN
Author Information
1. The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education of PRC, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
DNA Barcoding, Taxonomic;
methods;
DNA, Intergenic;
genetics;
DNA, Plant;
genetics;
Datura;
classification;
genetics;
Datura metel;
genetics;
Datura stramonium;
genetics;
Drug Contamination;
Flowers;
genetics;
Phylogeny;
Plants, Medicinal;
genetics;
Polymerase Chain Reaction;
Polymorphism, Single Nucleotide;
Sequence Analysis, DNA;
Solanaceae;
genetics;
Species Specificity
- From:
Acta Pharmaceutica Sinica
2011;46(11):1408-1412
- CountryChina
- Language:Chinese
-
Abstract:
To identify the original plant of Daturae Flos from its adulterants by DNA barcoding, the sequences of ITS2, psbA-trnH, matK, rbcL of four species including Datura metel, Darura innoxia, Darura stramonium and Brugmansia arborea were compared and analyzed. The PCR and sequencing success rate of the four regions (ITS2, psbA-trnH, matK, rbcL) was 100%, 90%, 100% and 85%, respectively. Sequences were assembled with CodonCode Aligner. K2P distances were calculated and NJ tree was performed by MEGA 4.1. Thirty SNPs were found among ITS2 sequences, and 33 insert/deletes were found among psbA-trnH intergenic regions. The interspecific K2P distance of ITS2 and psbA-trnH was obviously higher than that of the intraspecific one. As to matK and rbcL, there was no "Barcoding Gap" existing between inter- and intra-specific distances. The NJ trees of the four regions/combinations were built separately. Samples of Brugmansia arborea were clustered into one clade, and the other species of Datura L. formed another clade. The results showed that either ITS2 or psbA-trnH was useful to identify Daturae Flos from its adulterants.
