A preliminary study of anti-aging and wound healing of recombination cytoglobin.
- Author:
Zhao-Fa LI
1
;
Xiao-Fang ZHAO
;
Ting-Ting ZHANG
Author Information
1. Institute of Molecular Medicine, Ministry of Education, Huaqiao University, Quanzhou 362021, China. lizhaofa@hqu.edu.cn
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
Aging;
drug effects;
Alanine Transaminase;
metabolism;
Animals;
Antioxidants;
pharmacology;
Carbon Tetrachloride;
Catalase;
metabolism;
Cells, Cultured;
Chemical and Drug Induced Liver Injury;
metabolism;
Collagen;
Female;
Genetic Vectors;
Globins;
pharmacology;
Glutathione Peroxidase;
metabolism;
Humans;
Keratinocytes;
cytology;
metabolism;
L-Lactate Dehydrogenase;
metabolism;
Male;
Malondialdehyde;
metabolism;
Mice;
Oxidative Stress;
drug effects;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Recombinant Proteins;
pharmacology;
Superoxide Dismutase;
metabolism;
Wound Healing;
drug effects
- From:
Acta Pharmaceutica Sinica
2012;47(1):51-57
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, the preliminary study on antioxidant, enhancement of antioxidant enzymes activity, reducing the content of oxygen free radicals, delaying skin aging of the recombination cytoglobin (rCygb) purified by our lab were investigated through human keratinocyte cell line (HaCAT) H2O2 oxidative stress model, mouse skin aging model caused by continuous subcutaneous injection D-gal, rat acute liver injury model induced by CCl4 and rat skin wound healing model. The results showed that rCygb improved the activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), reduced the activities of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) as well as decreased the content of malondialdehyde (MDA). Skin biopsy showed that rCygb promoted angiogenesis, increased expression of collagen and improved the anti-inflammatory ability. All results displayed that rCygb improved the oxygen free radical scavenging ability, delayed skin aging and promoted wound healing.
