Simultaneous determination of five main index components and specific chromatograms analysis in Xiaochaihu granules.
- Author:
Yan-Shuang ZHUANG
1
;
Hao CAI
;
Xiao LIU
;
Bao-Chang CAI
Author Information
1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
administration & dosage;
chemistry;
Flavanones;
analysis;
Flavonoids;
analysis;
Glucosides;
analysis;
Glycyrrhetinic Acid;
analysis;
Quality Control;
Sensitivity and Specificity
- From:
Acta Pharmaceutica Sinica
2012;47(1):84-87
- CountryChina
- Language:Chinese
-
Abstract:
Reversed phase high performance liquid chromatography with diode array detector was employed for simultaneous determination of five main index components and specific chromatograms analysis in Xiaochaihu granules with a linear gradient elution of acetonitrile-water (containing 0.1% phosphoric acid) as mobile phase. The results showed that five main index components (baicalin, baicalein, wogonoside, wogonin, enoxolone) were separated well under the analytical condition. The linear ranges of five components were 0.518 - 16.576, 0.069 - 2.197, 0.167 - 5.333, 0.009 - 0.297 and 0.006 - 0.270 mg x g(-1), respectively. The correlation coefficients were 0.999 9, and the average recoveries ranged from 95% to 105%. Twelve common peaks were selected as the specific chromatograms of Xiaochaihu granules with baicalin as the reference peak. There were good similarities between the reference and the ten batches of samples. The similarity coefficients were no less than 0.9. The analytical method established is highly sensitive with strong specificity and it can be used efficiently in the quality control of Xiaochaihu granules.
