Localization of P2X7 Receptor Immunoreactivity in the Dorsal Root Ganglia of Guinea Pig
- Author:
Xuegong BAI
1
;
Ling JIANG
;
Zhenghua XIANG
Author Information
1. Xiangfan Hospital, Tongji Medical College, Huazhong University of Science and Technology
- Keywords:
P2X7 receptor;
ATP receptor;
purinoreceptor;
dorsal root ganglion;
NF200;
S100;
isolectin B4;
substance P
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(4):371-374,396
- CountryChina
- Language:Chinese
-
Abstract:
The P2X7 receptor mRNA and proteins in guinea-pig dorsal root ganglia (DRG) were studied by using RT-PCR and immunohistochemistry. The co-localization of P2X7 receptor with four cytochemical markers, the neurofilament protein NF200, S100, substance P and isolectin B4 (IB4) binding glyco-conjugates, were also examined. It was found that P2X7 receptor immunoreactivity (P2X7R-IR) was present mostly in large- and medium-sized DRG neurons (62 %±9 % and 36 %±6 % respectively in all P2X7R-IR neurons). All the P2X7R-IR neurons were also NF200 and S100 immunopositive. However, in a small number of NF200 or S100 immunopositive neurons no P2X7R-IR was detectable. All the IB4-positive or substance P-immunopositive neurons had no P2X7R-IR. These results demonstrate that P2X7 receptors are expressed in a large subpopulation of DRG neurons and they may play a role in the transduction of specific peripheral sensory signals.
