Effect of GSK-3 Overactivation on Neurofilament Phosphorylation
- Author:
Juan CHEN
1
;
Jie ZHOU
;
Youmei FENG
;
Jianzhi WANG
Author Information
1. Huazhong University of Science and Technology
- Keywords:
glycogen synthase kinase-3 (GSK-3);
neurofilament;
Alzheimer's disease
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(4):375-377,403
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we studied the effect of glycogen synthase kinase-3 (GSK-3) overactivation on neurofilament phosphorylation in cultured cells. After N2a cells were treated with the specific inhibitor (wortmannin) of phosphoinositol-3 kinase (PI-3K) or treated with wortmannin and the specific inhibitor (LiCl) of glycogen synthase kinase-3 (GSK-3), GSK-3 activity and neurofilament phosphorylation were detected by using GSK-3 activity assay, Western blots and immunofluoresence. Our results showed that after treatment of N2a cells with wortmannin for 1 h, overactivation of GSK-3 caused a reduced staining with antibody SMI32 and an enhanced staining with antibody SMI31. When N2a cells were treated with wortmannin and LiCl, the activity of GSK-3 was reduced substantially. At the same time, the phosphorylation of neurofilament was also reduced. The study demonstrated that overactivation of GSK-3 induced hyperphosphorylation of neurofilament and suggested that in vitro overactivation of GSK-3 resulted in neurofilament hyperphosphorylation and this may be the underlying mechanism for Alzheimer's disease.
