Effect of Trastuzumab in Combination with IFN α-2b on HER2 and MRP1 of ACHN
- Author:
Jiajing WANG
1
;
Zhiquan HU
;
Zhangqun YE
Author Information
1. Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
- Keywords:
renal cell carcinoma;
trastuzumab;
IFN α-2b;
HER2;
MRP1
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(3):326-328
- CountryChina
- Language:Chinese
-
Abstract:
Summary: To study the effect of Trastuzumab in combination with IFN α-2b on HER2 and MRP1 of ACHN in vitro, ACHN cell line of RCC was cultured by employing cell culture. The tetrazolium-based colorimetric assay was used to evaluate the growth-inhibiting effect of Trastuzumab with IFN α-2b. SP method was utilized to determine the expression of HER2 and MRP1 of the cells. Our results showed that Trastuzumab had inhibitory effect on the growth of renal tumor cells and reversing effect on the multi-drug-resistance (MDR) in RCC in a time- and dose-dependent manner. Treated with Trastuzumab with or without IFN α-2b, the expression of HER2 and MRP1 genes of RCC was decreased significantly (P<0.05). It was concluded that Trastuzumab with IFN α-2b could inhibit the proliferation of RCC and the expression of HER2 and MRP1 of ACHN and to some extent, reverse the MDR of the tumor cells.
