Study on the correlation between gene ponA/penA and the penicillin-resistance of Neisseria gonorrhoeae.
- Author:
Tie-Jun ZHANG
1
;
Xiao-Ming ZHOU
;
Tao ZHANG
;
Qing-Wu JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Bacterial Proteins; genetics; Base Sequence; DNA Mutational Analysis; DNA, Bacterial; Genes, Bacterial; Microbial Sensitivity Tests; Molecular Sequence Data; Mutagenesis, Insertional; Mutation; Neisseria gonorrhoeae; drug effects; genetics; Penicillin Resistance; genetics; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational
- From: Chinese Journal of Epidemiology 2007;28(9):901-905
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between penA/ponA and penicillin resistance of Neisseria gonorrhoeae.
METHODSAgar dilution method was used to determine the minimum inhibitory concentrations (MICs) of the strains. Polymerase chain reaction-single stand conformation polymerphism (PCR-SSCP) and PCR- restriction fragment length polymorphism (RFLP) were used to detect the mutations in ponA and penA genes, which encoding the penicillin binding protein-1 and -2 (PBP1 and PBP2), respectively.
RESULTSAll the 80 N. gonorrhoeae isolates had a D345 insertion detected in penA while 93.7% of N. gonorrhoeae isolates having a point mutation Leu421 --> Pro in ponA. Most of the penicillinase producing N. gonorrhoeae (PPNG) strains possessed the mutations in ponA and penA.
CONCLUSIONOur data suggested that the plasmid and chromosome mediated penicillin-resistance conjugately increased the level of resistance.
