Effect of recombinant human adenovirus p53 combined with cisplatin on the expression of human lung adenocarcinoma A549 cell gene.
- Author:
Jin-liang WANG
1
;
Shun-chang JIAO
;
Yi HU
;
Jin-yu LI
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; pathology; Adenoviruses, Human; genetics; Cell Line, Tumor; Cisplatin; pharmacology; Gene Expression Profiling; Genes, p53; genetics; Humans; Lung Neoplasms; metabolism; pathology; Transfection
- From: Acta Academiae Medicinae Sinicae 2010;32(4):383-388
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of recombinant human adenovirus p53 (rAd-p53) combined with cisplatin on the expression of human lung adenocarcinoma A549 cells.
METHODSHuman lung adenocarcinoma A549 cells were treated with rAd-p53 combined with cisplatin (combination group) or with cisplatin alone(cisplatin group). The expressions of the cell genes were compared between these two groups and the results were analyzed by SAM software.
RESULTA total of 43 differential genes were found, 15 of which were up-regulated and 28 were down-regulated.
CONCLUSIONFollowing introduction of rAd-p53, many genes regulating cell cycle, proliferation and apoptosis expresses up or down which significantly enhance chemosensitivity and killing efficiency of cisplatin on human lung adenocarcinoma A549 cells.
