Infection of human papillomavirus 16/18 DNA in patients with head and neck squamous cell carcinoma and its relationship with expression of Ki-67 and P53 protein.
- Author:
Jie-li ZHANG
1
;
Zhao SUN
;
Zhen HUO
;
Yu-feng LUO
;
Shui-qing MA
;
De-tian WANG
;
Jin-ling CAO
;
Ti YANG
;
Quan-cai CUI
;
Chun-mei BAI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Carcinoma, Squamous Cell; metabolism; virology; DNA, Viral; analysis; Female; Human papillomavirus 16; isolation & purification; Human papillomavirus 18; isolation & purification; Humans; Ki-67 Antigen; metabolism; Male; Middle Aged; Tumor Suppressor Protein p53; metabolism; Uterine Cervical Neoplasms; metabolism; virology
- From: Acta Academiae Medicinae Sinicae 2010;32(4):429-432
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the infection of human papillomavirus (HPV) 16/18 in patients with head and neck squamous cell carcinoma and explore the relationship between HPV infection and expressions of Ki-67 and P53 proteins in tumor tissue.
METHODThe level of HPV 16/18 DNA was measured by real time polymerase chain reaction, and Ki-67 and P53 proteins were measured by immunohistochemistry in tissues from head and neck squamous cell carcinoma.
RESULTSHPV 16/18 DNA was detected in 62.8% of our patients. In each cancer tissue sample, Ki-67 protein was expressed between 2% to 70%. P53 protein was expressed in 46.15% of our patients. No significant relation was found between HPV 16/18 DNA level and sex, smoking, drinking, and tumor clinical stages. However, level of HPV 16/18 DNA was found to have positive relation with tumor pathological grades and negative relation with P53 protein expression. No relation with Ki-67 protein expression was found.
CONCLUSIONHead and neck squamous cell carcinoma may be initiated by HPV 16/18 infection and the mechanism in carcinogenesis involves abnormal expression in P53 protein.
