Determination of ascitic bacterial 16S rRNA gene in the rapid diagnosis of spontaneous bacterial peritonitis.
- Author:
Hong-ying PAN
1
;
Hong-yun SUN
;
Cui-rong CHEN
;
Qun-wei CHEN
;
Jing XU
;
Rong-xia YE
;
Guo-qiang LOU
;
De-rong LU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Ascitic Fluid; microbiology; Bacterial Infections; diagnosis; DNA, Bacterial; analysis; Female; Humans; Male; Middle Aged; Peritonitis; diagnosis; microbiology; RNA, Ribosomal, 16S
- From: Acta Academiae Medicinae Sinicae 2010;32(5):557-560
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the value of ascitic bacterial 16S rRNA gene determination in the rapid diagnosis of spontaneous bacterial peritonitis (SBP).
METHODS16S rRNA gene from bacterial DNA in ascites was determined by quantitative fluorescent polymerase chain reaction (PCR) in 76 patients with suspected SBP and 6 patients with non-infectious ascites. The results were compared with those obtained from bacterial culture.
RESULTSThe positive rate of SBP was 22.4% among patients detected with ascitic bacterial 16S rRNA gene determination-based quantitative fluorescent PCR, which was significantly higher than that (7.9%) in patients only received bacterial culture (P<0.05). In addition,in 6 patients with non-infectious ascites,both the 16S rRNA gene determination-based quantitative fluorescent PCR and bacterial culture showed negative results.
CONCLUSIONS16S rRNA gene determination-based quantitative fluorescent PCR can be an effective tool for the rapid diagnosis of SBP. It is more sensitive than the bacterial culture.
