Expressions of myogenic markers in skeletal muscle differentiation of human bone marrow mesenchymal stem cells.
- Author:
Tai-yun LIU
1
;
Hong DAI
;
Jun LIN
;
Mei LI
;
Fu XIONG
;
Shan-wei FENG
;
Ya-ni ZHANG
;
Cheng ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biomarkers; Bone Marrow Cells; cytology; metabolism; Cell Differentiation; Cells, Cultured; Desmin; metabolism; Humans; Mesenchymal Stromal Cells; cytology; metabolism; Mice; Mice, Inbred mdx; Muscle, Skeletal; cytology; metabolism; MyoD Protein; metabolism; Myogenin; metabolism; Up-Regulation
- From: Acta Academiae Medicinae Sinicae 2010;32(5):516-520
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expressions of myogenic markers MyoD, myogenin,and desmin in skeletal muscle differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs).
METHODSMyogenic markers MyoD, myogenin,and desmin of hBM-MSCs cultured in vitro were detected by immunofluorescence and RT-PCR. A total of 21 8-to-10 week-old immunosuppressed mdx mice were transplanted with 1x107 passage 5 of hBM-MSCs. The mice were euthanized 2-24 weeks after transplantation,and gastrocnemius muscle were analyzed for human MyoD, myogenin,desmin,and dystrophin (Dys) expressions by immunohistochemistry and RT-PCR.
RESULTSThe numbers of MyoD-,myogenin-,and desmin-positive cells per 100 hBM-MSCs were 23.5∓5.3, 30.7∓6.2, and 28.4∓5.7, respectively. MyoD, myogenin, and desmin mRNA was observed in passage 5 of hBM-MSCs. After two weeks of hBM-MSCs transplantation,a small number of MyoD-and myogenin-positive cells were observed in skeletal muscle of mdx mice,and desmin-positive cells were observed 4 weeks after transplantation. Expressions of MyoD and myogenin were detected in the muscle of mdx mice 2-4 weeks after hBM-MSCs transplantation, which reached a peak 12-16 weeks later. Desmin was expressed in the muscle of mdx mice 4-8 weeks after transplantation,with much more expression after 16 weeks of transplantation. A small number of Dys-positive cell and Dys mRNA expression were presented in the muscle of mdx mice 4 and 8 weeks after hBM-MSCs transplantation,respectively. The expression of Dys in the muscle of mdx mice increased gradually after transplantation.
CONCLUSIONhBM-MSCs have the potential of myogenic differentiation in vitro and contribute to myogenic conversion in xenogeneic animal,during which the up-regulation of MyoD and myogenin expressions may play an important role.