Effects of newborn bull serum and vitamins on cryopreservation of mouse seminiferous epithelial cells.
- Author:
Lian-Jun LI
1
;
De-Xue LI
;
Xue-Ming ZHANG
;
Zhan-Peng YUE
;
Xing-Hao WEN
;
Bing-Kun LUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Ascorbic Acid; pharmacology; Cattle; Cryopreservation; Epithelial Cells; physiology; Fetal Blood; physiology; Male; Mice; Seminiferous Epithelium; cytology; Vitamin K; pharmacology
- From:National Journal of Andrology 2002;8(4):244-246
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate the effects of newborn bull serum(NBS), vitamin C and vitamin E on cryopreservation of mouse seminiferous epithelial cells.
METHODSThe seminiferous epithelial cells from 7-day-old mice were cryopreserved in different freezing solutions. The cell recoveries were examined by Trypan blue exclusive staining after thawing. The freezing solutions composed of DMEM, 10% dimethylsulphoxide(DMSO), and 0, 5%, 10%, or 20% NBS, respectively, or composed of DMEM, 10% DMSO, 10% NBS, and 150 micrograms/ml vitamin C or 50 micrograms/ml vitamin E, respectively.
RESULTSThe cell recoveries in freezing solution containing 0, 5%, 10%, or 20% NBS were 83.4%, 84.7%, 85.7% and 83.6%, respectively. There were no significant differences between them. The cell recoveries in freezing solution containing vitamin C or vitamin E were 88.0% and 82.9%, respectively. There was no significant differences compared with that in freezing solution containing 10% DMSO and 10% NBS.
CONCLUSIONSNBS, vitamin C and vitamin E have no significant protecting effects on mouse seminiferous epithelial cells, and can not significantly improve the cell recoveries.
