The expression of nitric oxide synthase in testes of male rat.
- Author:
Mei-Xiang LI
1
;
Li-Ping HE
;
Zi-Qian GUO
;
Yue-Shun LIU
;
Zhi-Feng LONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Immunohistochemistry; Male; Nitric Oxide; biosynthesis; Nitric Oxide Synthase; analysis; Rats; Rats, Sprague-Dawley; Testis; enzymology
- From:National Journal of Andrology 2002;8(4):250-252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo determine the expression of nitric oxide synthase (NOS) in testis and to investigate the effects of NO on the reproductive function of testis.
METHODSTestes of adult male Sprague-Dawley rats were fixed in 4% paraformaldehyde. The paraffin sections were made as routine. Immunohistochemical ABC method was used to observe the localization of NOS.
RESULTSEndothelia NOS (eNOS), neuronal NOS (nNOS) and inductive NOS (iNOS) were all expressed in Leydig cells. Only eNOS was expressed in peritubular myoid cells, endothelial and smooth muscle cells of blood vessel, while only nNOS expressed in tunica adventitia of testicular blood vessels. The reactive substance distributes in cytoplasm with negative nuclei. Immunoreactivity for eNOS, nNOS and iNOS in all spermatogenic cells was negative.
CONCLUSIONSThree kinds of NOS were all expressed in testis and the distribution of different NOS had a little difference.
