Influence of dibutyryl cyclic adenosine monophosphate on motility of sperm.

Author: Yang LI ¹ ; Ji-Hong LIU
Author Information

1. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Publication Type:Journal Article
MeSH: Adult; Bucladesine; pharmacology; Cell Survival; drug effects; Dose-Response Relationship, Drug; Humans; Male; Sperm Motility; drug effects; Spermatozoa; cytology; drug effects
From: National Journal of Andrology 2002;8(6):398-400
CountryChina
Language:Chinese
Abstract:
OBJECTIVESTo investigate the influence of cAMP/PKA signal transduction on human sperm motility, and to study the effect of dibutyryl cyclic adenosine monophosphate dibutyryl cyclic adenosine monophosphate (dbcAMP) on human sperm motility in vitro.
METHODSSperm aseptically obtained by masturbation and prepared by swim-up technique from 10 healthy fertile men were incubated with different concentrations of dbcAMP. Measurement of mobility were carried out at 20, 30, and 60 min in all specimens.
RESULTSThe sperm treated with dbcAMP showed a significant increase in sperm progressive motility and the percentage of motile cells. The effect seemed enhanced with the increasing of dbcAMP concentration. VSL and VCL were not affected by dbcAMP.
CONCLUSIONSdbcAMP can activate the mobility of human sperm in vitro.