Effect of spinal cord injury on iNOS and Bcl-2 gene expression in spermatogenic cell of the male rat.
- Author:
Yong WEI
1
;
Xiao-Bin ZHANG
;
Jie ZHANG
;
Fan CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Male; Nitric Oxide Synthase; biosynthesis; genetics; Nitric Oxide Synthase Type II; Proto-Oncogene Proteins c-bcl-2; biosynthesis; genetics; Rats; Rats, Sprague-Dawley; Spinal Cord Injuries; metabolism
- From: National Journal of Andrology 2003;9(1):28-54
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate the cause of spermatogenic cells decreasing after spinal cord injury.
METHODSImmunohistochemical S-P method was used to detect the expression of iNOS and Bcl-2 protein in the testis of spinal cord injured and sham-operated adult rats in second week and fourth week after operation.
RESULTSSecond week and fourth week after operation, the expression of iNOS of operated rats increased significantly (P < 0.05). Fourth week after operation, the number of Bcl-2 positive cells in spinal cord injured group decreased significantly (P < 0.05).
CONCLUSIONSThe expression veition of iNOS and Bcl-2 is the cause that inducing the decrease of spermatogenic cells in testis of spinal cord injured rats.