Changes of 5 alpha-reductase type II activity in sexual gland of diabetic male rats.
- Author:
Hong-Jun WANG
1
;
Jia-Ying ZHANG
;
Su-Huan LIU
;
Hui ZHAO
;
Zhong-Shan WANG
Author Information
- Publication Type:Journal Article
- MeSH: 3-Oxo-5-alpha-Steroid 4-Dehydrogenase; metabolism; Animals; Diabetes Mellitus, Experimental; enzymology; Epididymis; enzymology; Male; Prostate; enzymology; Rats; Rats, Wistar; Testis; enzymology
- From: National Journal of Andrology 2003;9(2):82-84
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo study the changes of sexual gland 5 alpha-reductase type II activity in pubertal and adult rats with diabetes.
METHODSWe selected 40 and 90 days old male Wistar rats as pubertal and adult animal model respectively, 30 rats in each group. The rats were randomly divided into three groups: control group (C), diabetic group (D) and diabetes with insulin replacement group (ID). The activity of 5 alpha-reductase type II was measured with thin layer chromatography in the epididymis, prostate and testis.
RESULTS1. In all sexual glands of pubertal rats, the activity of 5 alpha-reductase type II in D group is significantly lower than that in C and ID groups. 2. In all sexual glands of adult rats. there is no difference in the activity of 5 alpha-reductase type II among these groups.
CONCLUSIONSThe activity of 5 alpha-reductase type II is likely to be influenced by metabolic environment, hormonal levels and local specific factors in pubertal rats, but it is relatively stable in adult rats.
