Application of two-dimensional electrophoresis and mass spectrometry in the research of testicular proteins.
- Author:
Ye-Fei ZHU
1
;
Xiang MA
;
Zuo-Min ZHOU
;
Jia-Hao SHA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Electrophoresis, Gel, Two-Dimensional; Male; Mass Spectrometry; Mice; Mice, Inbred ICR; Proteins; analysis; Testis; chemistry
- From: National Journal of Andrology 2003;9(2):85-89
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo evaluate the application of two-dimensional electrophoresis and mass spectrometry in the research of proteins expressed in testis.
METHODSProtein from adult ICR mouse testes was extracted by two-dimensional electrophoresis. Two protein spots were cut from the gel, then the proteins were digested in-gel by enzyme and the generated peptides were measured by matrix assisted laser desorption/ionization-time of flight mass spectrometry. The proteins were identified through database searching.
RESULTSTwo spots in Commasie Brilliant Blue-stained gel were identified as serum albumin and protein disulfide isomerase by database searching.
CONCLUSIONSThis rapid high resolution and efficient method is a very powerful way to analyze testicular proteins.