The effect of the period of cryopreservative storage on the cryosurvival of human spermatozoa.
- Author:
Jin-Xia MA
1
;
Li-Xin QIAN
;
Tian-Hua JIANG
;
Qiang LU
;
Hong-Fei WU
Author Information
- Publication Type:Journal Article
- MeSH: Cryopreservation; Humans; Male; Semen Preservation; Sperm Banks; Spermatozoa; physiology; Time Factors
- From: National Journal of Andrology 2003;9(2):122-123
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo study the effect of cryopreservative period on the cryosurvival of human spermatozoa and find out the optimal recovery time of cryopreservation.
METHODSEighty-eight semen samples were collected from normal donors and divided randomly into 5 groups according to the period of cryopreservative storage (1 d, 7 d, 30 d, 180 d, 300 d) in liquid nitrogen after being frozen by the programized, three-step freezing method. Fresh and frozen-thawed semen were examined by the routine analysis of semen and then the sperm recovery rate were calculated.
RESULTSThere were no significant differences in sperm recovery rate between group I and the others (P > 0.05). The period of cryopreservative storage in liquid nitrogen had no correlation with the cryosurvival of human spermatozoa (r = 0.05, P > 0.05).
CONCLUSIONSIt was indicated that freezing-thawing after 24 h would be helpful to the screening of semen donors in batches for donor insemination of human sperm bank.
