Facial paralysis induced by herpes simplex virus type 1 and the study of facial neurons apoptosis in mice
10.3760/cma.j.issn.1673-0860.2011.09.010
- VernacularTitle:小鼠病毒性面神经炎模型中面神经元凋亡的研究
- Author:
Wen LIU
1
;
Zhi-Qiang GAO
;
Ping SHEN
;
Guo-Dong FENG
;
Yang ZHA
;
Yan-Dong ZHANG
;
Pei-Mao LIU
Author Information
1. 徐州医学院附属医院
- Keywords:
Herpesvirns 1,human;
Bell palsy;
Motor neurons;
Apoptosis;
bcl-2-associated X protein;
Disease models,animal
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2011;46(9):742-746
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the apoptosis of facial motor neurons and the expression of apoptosis-related genes, Bcl-2 and Bax, in the animal model of viral facial paralysis. Methods Total of 84Balb/c mice were divided into viral inoculation group and nerve transaction group. The animals were executed 1, 3, 7, 10, 15, 20 and 30 days after being operated respectively. The histopathological features of facial neurons in brain stem were observed by HE and Nissl stain. The changes of facial neuronal apoptosis were observed by TUNEL The changes of expression of Bcl-2 and Bax genes in facial neurons were observed by immunohistochemistry staining. Results After nerve transection, increased apoptotic cells were found in homolateral facial motor neucleus and the peak appeared at 10 and 15 days. The level of Bcl-2 expression inneurons declined while the expression of Bax increased gradually. Correspondingly, the ratio of Bcl-2/Bax declined. In the viral inoculation group, no visible change of apoptosis and Bax expression, but the level of Bcl-2 and the ratio of Bcl-2/Bax increased gradually. Conclusions Comparing to axotomy, facial motor neucleus in HSV-1 infective animal model are free of apoptosis. Both the mild form of lesion and the ability to block apoptosis of HSV-1 are likely to be involved into the phenomenon. Bcl-2 and Bax might interfere with the apoptotic response.
