Effects of combining tumor necrosis factor related apoptosis-inducing ligand with PI3-K-Akt inhibition on nasopharyngeal carcinoma cell
10.3760/cma.j.issn.1673-0860.2011.10.013
- VernacularTitle:联合肿瘤坏死因子相关凋亡诱导配体和阻断PI3-K-Akt信号通路影响鼻咽癌细胞生长凋亡的实验研究
- Author:
Shi-Sheng LI
1
;
Qing-Lai TANG
;
Shu-Hui WANG
;
Yue-Hong CHEN
;
Jia-Jia LIU
;
Shuang WANG
;
Xin-Ming YANG
Author Information
1. 中南大学湘雅二医院
- Keywords:
TNF-related apoptosis-inducing ligand;
Chromones;
Morpholines;
1-Phosphatidylinositol 3-kinase;
Apoptosis;
Nasopharyngeal neoplasms
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2011;46(10):854-858
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of combinative therapy of tumor necrosis factor related apoptosis-inducing ligand (TRAIL) and PI3-K-Akt inhibitor on the growth and apoptosis of nasopharyngeal carcinoma (NPC) cells and underlying mechanisms.Methods With cell growth assay,flow cytometric analysis and Western blotting,the effects of TRAIL and PI3-K-Akt special inhibitor ( LY294002 ) on cell growth,apoptosis and related proteins expressions in CNE-2 cell lines were studied.Results When concentrate of TRAIL ＞ 1 ng/ml,viability rate of cells in combinative treatment group with TRAIL and LY294002 was higher than that in the single treatment group with TRAIL ( all P ＜ 0.05 ).When concentrate of TRAIL were 10 ng/ml and 100 ng/ml,the combinative treatment induced CNE-2 apoptosis more obviously than single treatments ( t were 7.167 and 7.206,all P ＜ 0.05 ).The combination group showed more cleavage of Caspase-8,Caspase-3,Caspase-9 than single treatment groups.Conclusions Combinative application of TRAIL and PI3-K-Akt pathway inhibitor inhibits the growth of CNE-2 and induces apoptosis.The mitochondrial dependent pathway is implicated for the underlying mechanism.
