Distribution and clonality of T cell receptor Vγ and Vδ subfamily in peripheral blood of patients with allergic rhinitis before and after immunotherapy
10.3760/cma.j.issn.1673-0860.2011.12.006
- VernacularTitle:变应性鼻炎免疫治疗前后外周血T细胞抗原识别受体Vγ和Vδ亚家族谱系和克隆性分析
- Author:
Qin-Tai YANG
1
;
Xue-Kun HUANG
;
Peng LI
;
Yu-Lian CHEN
;
Ge-Hua ZHANG
Author Information
1. 中山大学附属第三医院
- Keywords:
Rhinitis,allergic,perennial;
Immunotherapy;
Receptors,antigen,T cell,gamma-delta;
Pedigree clonality
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2011;46(12):992-997
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution and clonality of T cell receptor(TCR)Vγand Vδ subfamily in peripheral blood of patients with allergic rhinitis before and after 1 year treatment with immunotherapy.Methods The specific IgE and the complementary determinant region 3(CDR3)of TCR V γ(Ⅰ-Ⅲ)and Vδ(1-8)subfamily genes in mononuclear cells were amplified from 10 effective cases of allergic rhinitis before and after 1 year treatment with immunotherapy,to observe the distribution and utilization of TCR Vγ and Vδ repertoire.The positive PCR products were further labeled with RT-PCR and analyzed by gene scan technique to determine the CDR3 size and evaluate the clonality of the detectable TCR Vγ and Vδ T cells.Peripheral blood of 10 healthy adults served as controls.Results All symptoms were significantly improved after 1 year specific iammanotherapy,but no changes were seen in specific IgE [(22.89 ±9.60)kU/L before treatment,(19.62 ± 7.63)kU/L after treatment,Z =1.051,P ＞ 0.05].No statistically significant differences of expression levels of the TCR Vγ Ⅰ-Ⅲ subfamily genes were found between patients with allergic rhinitis normal control group(t value were-0.679,-0.516,-0.808,all P ＞ 0.05),but significantly decreased after 1 year treatment.There were statistically significant differences of expression levels of the TCR VγⅠ-Ⅱ subfamily genes before and after treatment(t value were-2.904,-2.217,all P ＜ O.05).5.30 ± 0.82,4.90 ± 0.57 and 5.20 ± 1.40 out of TCR Vδ(1-8)subfamilies were selectively expressed in T cells in patients with allergic rhinitis before and after 1 year treatment and normal control group,predominantly for TCR Vδ 1,2,3 and 6.The TCR Vδ 6 subfamily was found to have statistically significant differences in these groups(Fisher′s Exact Test,P ＜ 0.05).Compared with the normal control group and the allergic rhinitis group before treatment,a significant higher frequency of Vδ 6 oligoclonal was identified in T cells in patients with allergic rhinitis after 1 year treatment.Conclusions There was difference in the expression levels of the TCR Vγ Ⅰ-Ⅲ subfamily genes and distribution and clonality of TCR Vγand Vδ subfamily T cells in peripheral blood of patients with allergic rhinitis before and after 1 year treatment.Specific immunotherapy can be effective in alleviation of the symptom in patients with allergic rhinitis during the early stage,possibly by inducing TCR γδ T cells,especially the TCR Vδ6 subfamily,and possibly no significant relativity between symptom and specific IgE.
