Influence of carbon monoxide on the expression of adhesion molecules stimulated with tumor necrosis factor-alpha and interleukin-1beta on human gingival fibroblasts.
- Author:
Huaqiang ZHAO
1
;
Lingling WEI
;
Meng HOU
;
Pingping MU
;
Fengcai WEI
;
Hui SONG
;
Pishan YANG
Author Information
- Publication Type:Journal Article
- MeSH: Carbon Monoxide; Cells, Cultured; Fibroblasts; Gingiva; Humans; Intercellular Adhesion Molecule-1; Interleukin-1beta; NF-kappa B; RNA, Messenger; Tumor Necrosis Factor-alpha; Vascular Cell Adhesion Molecule-1
- From: West China Journal of Stomatology 2012;30(3):304-313
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of carbon monoxide on the expression of adhesion molecules stimulated by inflammatory cytokines on human gingival fibroblasts.
METHODSHuman gingival fibroblasts were stimulated with 50 ng x mL(-1) tumor necrosis factor (TNF)-alpha and 10 ng x mL(-1) interleukin (IL)-1beta concurrently in the presence or absence of 500 micromol x L(-1) carbon monoxide releasing molecule (CORM). Expression of intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM)-1 at protein and mRNA level was examined by Western blot and reverse transcription polymerase chain reaction (RT-PCR), respectively. Activity of transcription factor NF-kappaB was evaluated by reporter gene assay.
RESULTSExpression of ICAM-1 and VCAM-1 on human gingival fibroblasts increased dramatically after concurrent stimulation of TNF-alpha and IL-1beta, while CORM inhibited the upregulation of ICAM-1 and VCAM-1. CORM decreased the activity of NF-KB stimulated by TNF-alpha and IL-1beta.
CONCLUSIONCarbon monoxide could be a promising way in treating of periodontitis.
