Effects of intracellular osmolality changes on the voltage-gated sodium channels currents of trigeminal ganglion neuron.
- Author:
Jiefei SHEN
1
;
Haiye WANG
;
Yingyi MA
;
Yunfei LIU
;
Shuyuan ZHANG
;
Li DU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Membrane Potentials; Neurons; Osmolar Concentration; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley; Sodium Channels; Trigeminal Ganglion
- From: West China Journal of Stomatology 2012;30(4):338-342
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the biomechanical effects of intracellular changes on the voltage-gated sodium channels (VGSCs) on trigeminal ganglion neuron (TRGN).
METHODSTRGN cells were acutely isolated from the neonatal SD rats. The voltage-dependent currents of the VGSCs on these neurons were elicited and analyzed by whole-cell patch-clamp recordings and the intracellular anisotonicity stimuli was established by adjusting the content of pipette solution. The effects of hypo-(260 mOsm) and hypertonic (350 mOsm) osmolarity on the activation and inactivation kinetics of VGSCs on TRGN were evaluated, compared with the normal intracellular environment.
RESULTSThe results demonstrated that intracellular hypotonic stimuli could influence both the activation and inactivation characteristics of VGSCs currents, including the membrane potential at half inactivation (V0.5) of the G-V and inactivation curves had obvious statistics significance (P<0.05) between hypotonicity (260mOsm) and isotonicity (306mOsm). However, only inactivation properties changed under intracellular hypertonic effects, including inactivation rate and k value.
CONCLUSIONIt suggests that the kinetics of VGSCs on TRGN can be modulated both by intracellular hypo- and hypertonic with different characteristics.
