Screen and analysis of differentially expressed genes related to stem-like cells in tongue squamous cell carcinoma Tca8113 cell line.

Bo ZOU; Ping JI; Shoujuan SUN; Xiaojuan QI

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Screen and analysis of differentially expressed genes related to stem-like cells in tongue squamous cell carcinoma Tca8113 cell line.

Author: Bo ZOU ¹ ; Ping JI ; Shoujuan SUN ; Xiaojuan QI
Author Information

1. Dept. of Oral and Maxillofacial Surgery, Hospital of Stomatology, Chongqing Medical University, Chongqing Research Center for Oral Diseases and Biomedical Science, Chongqing 401147, China.
Publication Type:Journal Article
MeSH: Carcinoma, Squamous Cell; Cell Line; Humans; Nucleic Acid Hybridization; Tongue Neoplasms
From: West China Journal of Stomatology 2012;30(5):458-462
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo screen the differentially expressed genes of stem-like cells in tongue squamous cell carcinoma using suppression subtractive hybridization (SSH).
METHODSThe cells with high and low expression of aldehyde dehydrogenase(ALDHbr and ALDHlow) were isolated respectively from human tongue squamous cell carcinoma Tca8113 cell line. SSH was used to identify differentially expressed genes in those two groups. Positive cell clones with high/ low-expressed ALDH were screened randomly for the genetic sequences and function analysis by the bioinformatics technology.
RESULTSTotal 62 differentially expressed genes were identified, most of which got involved in regulation of cell cycle, cellular differentiation and other biology activities as gene ontology (GO) analysis indicated. Signal pathway analysis revealed 12 pathways related to 11 genes changed significantly.
CONCLUSIONSeveral potential novel biomarker candidates of tongue cancer stem cells were identified and they may provide important insights into the functions and characteristics of human tumor stem cell.