Protein-protein interaction network of receptor activator of nuclear factor-kappaB ligand and macrophage colony-stimulating factor induced differentiation of osteoclasts.

Author: Pingxiu ZHOU ¹ ; Ji'an HU ; Xiangyong MENG
Author Information

1. Dept. of Oral Pathology, Stomatology Hospital, Zhejiang University College of Medicine, Hangzhou 310006, China.
Publication Type:Journal Article
MeSH: Animals; Carrier Proteins; Cell Differentiation; Macrophage Colony-Stimulating Factor; Membrane Glycoproteins; Mice; Osteoclasts; Protein Interaction Maps; RANK Ligand; Receptor Activator of Nuclear Factor-kappa B
From: West China Journal of Stomatology 2012;30(5):518-521
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo systemically investigate receptor activator of nuclear factor-kappaB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) induced differentiation of osteoclasts.
METHODSMouse protein-protein interaction(PPI) database NIA and published microarray dataset GES16749 were used to construct and analyze PPI network of RANKL and M-CSF induced mouse monocyte RAW264.7.
RESULTSIn the PPI network, transforming growth factor beta receptor 1 (TGFBR1), Rous sarcoma oncogene (SRC), myelocytomatosis oncogene(MYC) and integrin beta 3 (ITGB3) were able to interact with more proteins and they were the key nodes in the signaling transduction.
CONCLUSIONTGFBR1, SRC, MYC and ITGB3 might be the key points of RANKL and M-CSF induced differentiation of osteoclasts.