Relationship between endothelial nitric oxide synthase Glu298Asp gene polymorphisms and the chronic periodontitis with type 2 diabetes mellitus.
- Author:
Haijuan HOU
1
;
Fan ZHANG
;
Meilin ZHAO
;
Guifen CAO
;
Haiyun HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Chronic Periodontitis; Diabetes Mellitus, Type 2; Gene Frequency; Genotype; Humans; Male; Middle Aged; Nitric Oxide Synthase Type III; Polymerase Chain Reaction; Polymorphism, Genetic; Risk Factors
- From: West China Journal of Stomatology 2012;30(6):628-631
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship between endothelial nitric oxide synthase (eNOS) Glu298Asp gene polymorphism and chronic periodontitis (CP) with type 2 diabetes mellitus (T2DM).
METHODSDNA from patients' buccal swabs of CP, CP with T2DM, T2DM and health was isolated and extracted. The eNOS Glu298Asp gene polymorphism were assessed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) respectively.
RESULTSThe distribution of eNOS Glu298Asp genotype in CP group, T2DM group, CP with T2DM group and health group showed statistical differences (chi2 = 18.503, P = 0.005), and the gene frequency showed statistical differences (chi2 = 8.243, P = 0.041). Compared health group to CP with T2DM group, OR value of the genetype T was 0.962, 95% confidence interval lay in between 0.737 to 1.256, which showed T maybe a protective factor. While OR value of the genetype G was 1.043, 95% confidence interval lay in between 0.781 to 1.391, which showed G maybe a risk factor. However, neither T nor G was statistically significant.
CONCLUSIONBased on these findings, there are some association between eNOS Glu298Asp polymorphism and the risk of CP group, T2DM group, CP with T2DM group.
