Theanine improves the function of dendritic cells via the downregulation of cyclooxygenase-2 expression.

Mingsheng Lei; Jianhong Zuo; Min LI; Qihua GU; Chengping HU

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Theanine improves the function of dendritic cells via the downregulation of cyclooxygenase-2 expression.

Author: Mingsheng LEI ^{1
,

2} ; Jianhong ZUO ³ ; Min LI ⁴ ; Qihua GU ⁴ ; Chengping HU ⁵
Author Information

1. Department of Respiratory and Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China
2. Department of Respiratory and Critical Care Medicine, Zhangjiajie City Hospital, Zhangjiajie, Hunan 427000, China.
3. Medical School, University of South China, Hengyang, Hunan 421001, China.
4. Department of Respiratory and Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
5. Department of Respiratory and Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China. Email: huchengp28@yahoo.com.cn.
Publication Type:Journal Article
MeSH: Bombesin; pharmacology; Cells, Cultured; Cyclooxygenase 2; metabolism; Dendritic Cells; drug effects; metabolism; Enzyme-Linked Immunosorbent Assay; Glutamates; pharmacology; Humans; Interleukin-10; metabolism; Interleukin-12; metabolism
From: Chinese Medical Journal 2014;127(8):1545-1549
CountryChina
Language:English
Abstract:
BACKGROUNDTumor cells can reduce the number of dendritic cells (DCs) in the tumor environment and cause DC dysfunction through autocrine or paracrine pathways. We sought to measure cyclooxygenase-2 (COX-2) expression in bombesin-inhibited DCs treated with theanine in vitro and to explore the protection and activation effects of theanine on DCs.
METHODSEnzyme-linked immunosorbent assay (ELISA), reverse transcription-polymerase chain reaction (RT-PCR), and Western blotting were used to analyze the effects of theanine on COX-2 expression and interleukin (IL)-12/IL-10 secretion of bombesin-treated DCs.
RESULTSDCs acquired an impaired phenotype as a result of bombesin treatment. Theanine increased the expression of mature DC surface molecules. The number of cell apoptosis with the treatment of bombesin and theanine significantly decreased, accounting for 15.9%, compared with 26.1% of cell apoptosis with bombesin. COX-2 expression in bombesin-treated DCs was inhibited by theanine in a dose-dependent manner. Theanine promoted DC secretion of IL-12. IL-12 levels reached (137.4 ± 4.9) pg/ml with theanine at 200 µmol/L. However, theanine inhibited the secretion of IL-10 in a dose-dependent manner. IL-10 levels were only (58.4 ± 6.9) pg/ml with theanine at 200 µmol/L.
CONCLUSIONTheanine inhibits the transcription and translation of COX-2 and regulates the balance of IL-10/IL-12 secretion in bombesin-inhibited DCs, leading to the recovery of a state of activation in DCs.