High K-induced Epileptiform Activity under GABA Antagonist and Carbamazepine in the Rat Visual Cortex.
- Author:
Byung Jun CHOI
1
Author Information
1. Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea. choibj@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
K;
Carbamazepine
- MeSH:
6-Cyano-7-nitroquinoxaline-2,3-dione;
Animals;
Bicuculline;
Brain;
Carbamazepine*;
GABA Antagonists*;
gamma-Aminobutyric Acid*;
Magnesium;
Models, Theoretical;
Rats*;
Rats, Sprague-Dawley;
Synapses;
Visual Cortex*
- From:
Journal of the Korean Child Neurology Society
2006;14(1):16-23
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The goal of this study was to investigate the effect of increase of K on the excitability of the visual cortex under GABA antagonist, carbamazepine(CBZP), 6-cyano-7-nitroquinoxaline-dione disodium(CNQX) and D-(-)-2-amino-5-phosphonopentanoic acid(D-AP5) on the increase of K-induced epileptiform activity. METHODS: The visual cortex slices were obtained from Sprague-Dawley rats. Extracellular recordings were performed to observe the induction of epileptiform discharges perfused by artificial CSF(ACSF) with an increase of K and the effect of 0.5 and 1 mM CBZP, 10 micrometer CNQX and 50 micrometer D-AP5 on the increase of K-induced epileptiform activity. RESULTS: Spontaneous epileptiform activities were increased by ACSF with 10 mM K under bicuculline compared to 4 mM K. CBZP blocked and 10 micrometer CNQX and 50 micrometer D-AP5 decreased the 10 mM K-induced spontaneous epileptiform activity. CONCLUSION: An increase of K augmented spontaneous epileptiform activity. This experimental model increased the excitability of the brain, which may be influenced by GABA. CBZP blocked the increase of K-induced epileptiform activity, which showed the increase of excitability. An increase of K-induced epileptiform activity was decreased by CNQX and D-AP5, which may show the attenuation of voltage-dependent low magnesium effect and decrease the excitability in terms of synapse induction.
