Effect of mitochondrial DNA deletion on growth and invasiveness of human lymphoma Namalwa cells.
- Author:
Jia MA
1
;
Qing ZHANG
;
Changjie CHEN
;
Qingling YANG
;
Sulian CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Calcium; metabolism; Cell Division; Cell Line, Tumor; Cell Movement; Cyclooxygenase 2; metabolism; DNA, Mitochondrial; genetics; Humans; Lymphoma; genetics; pathology; Reactive Oxygen Species; metabolism; Sequence Deletion
- From: Journal of Southern Medical University 2013;33(2):188-192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of mitochondrial DNA (mtDNA) deletion on the growth and invasiveness of human lymphoma Namalwa cells.
METHODSρ(0)-Namalwa cells with mtDNA deletion were generated by treating Namalwa cells with ethidium bromide and confirmed by selective ρ(0) test medium analysis, PCR and Western blotting. The growth of ρ(0)-Namalwa cells was evaluated by MTT assay and cell cycle analysis, and the cell migration and invasiveness were assessed with Transwell assay. Reactive oxygen species (ROS) production and cytosolic Ca(2+) were detected by flow cytometry.
RESULTSρ(0)-Namalwa cells could grow and divide normally in selective medium supplemented with uridine and pyruvate but not in nonselective medium. PCR did not yield the products of mtDNA, nor was COXII expression detected in ρ(0)-Namalwa cells. ρ(0)-Namalwa cells showed an obvious attenuation of cell proliferation and migration abilities with significantly lowered ROS production and cytosolic Ca(2+).
CONCLUSIONThe suppressed growth and migration of ρ(0)-Namalwa cells may be the result of decreased ROS production and cytosolic Ca(2+).
