CD99 regulates redifferentiation of classical Hodgkin's lymphoma cell line L428 towards B cells.
- Author:
Xinhua ZHOU
1
;
Xueping HUANG
;
Zuoping HUANG
;
Zhiqiang WANG
;
Tong ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: 12E7 Antigen; Antigens, CD; genetics; B-Lymphocytes; cytology; Cell Adhesion Molecules; genetics; Cell Differentiation; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Hodgkin Disease; genetics; pathology; Humans
- From: Journal of Southern Medical University 2013;33(2):235-238
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of CD99 overexpression on the morphology and differentiation-related phenotypes of classical Hodgkin's lymphoma cell line L428 and investigate the role of CD99 gene in Hodgkin/Reed-Sternberg (HRS) cell generation and transformation.
METHODSThe effect of CD99 overexpression on the cell morphology was detected by HE staining and phalloidin staining. Differentiation-related protein expressions were detected by immunocytochemistry and flow cytometry after stable transfection of CD99 gene in L428 cells.
RESULTSCD99 overexpression caused a decrease of the cell size and reorganization of the actin cytoskeleton in L428 cells. Upregulation of CD99 led to the loss of classical Hodgkin's lymphoma diagnosis marker CD30 and CD15 and the restoration of the B-cell makers of PAX5, CD19, CD79α, BCL-6, and CD10.
CONCLUSIONCD99 overexpression leads to redifferentiation of L428 cells towards B cells, suggesting that the loss of B-cell phenotype in classical Hodgkin's lymphoma is very likely a result of down-regulated CD99 expression.
