Histone acetylation modulates influenza virus replicative intermediate dsRNA-induced interleukin-6 expression in A549 cells.
- Author:
Bikui TANG
1
;
Shouwei WU
;
Mingjie HU
;
Xiaofen LIU
Author Information
- Publication Type:Journal Article
- MeSH: Acetylation; Cell Line, Tumor; Gene Expression Regulation; Histone Deacetylase Inhibitors; pharmacology; Histones; metabolism; Humans; Interleukin-6; metabolism; Orthomyxoviridae; genetics; metabolism; Promoter Regions, Genetic; RNA, Double-Stranded; RNA, Messenger; genetics; RNA, Viral
- From: Journal of Southern Medical University 2013;33(2):282-286
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of histone acetylation in regulating influenza virus replicative intermediate double-stranded RNA (dsRNA)-induced interleukin-6 (IL-6) expression in A549 cells.
METHODSA549 cells were treated with influenza virus replicative intermediate dsRNA, histone deacetylase (HDAC) inhibitor trichostatin A (TSA), or HADC small interfering RNA (siRNA). The changes in the cellular IL-6 promoter activities were detected by dual-luciferase assay, and IL-6 mRNA and protein expressions in the cells were determined using real-time RT-PCR and ELISA, respectively.
RESULTSInfluenza virus replicative intermediate dsRNA obviously up-regulated IL-6 expression in the cells. HDAC inhibitor TSA significantly enhanced the activity of IL-6 promoter and increased IL-6 mRNA expression in A549 cells, and HDAC3 may play an important role in this process. HDAC inhibitor TSA and DNMT inhibitor DAC showed no synergic effect in regulating IL-6 expression.
CONCLUSIONSInfluenza virus replicative intermediate dsRNA-induced IL-6 expression in A549 cells is regulated by histone acetylation.
