Advanced oxidation protein products inhibit proliferation and differentiation of rat osteoblasts through oxidative stress.
- Author:
Nan SUN
1
;
Li YANG
;
Yingbin LI
;
Yanzhen CHENG
;
Zhen ZHANG
;
Dehong CAI
Author Information
- Publication Type:Journal Article
- MeSH: Advanced Oxidation Protein Products; pharmacology; Alkaline Phosphatase; metabolism; Animals; Cell Differentiation; Cell Proliferation; drug effects; Cells, Cultured; Collagen Type I; metabolism; Osteoblasts; cytology; drug effects; Oxidative Stress; drug effects; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; metabolism; Receptor for Advanced Glycation End Products; Receptors, Immunologic; metabolism
- From: Journal of Southern Medical University 2013;33(3):356-359
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of advanced oxidation protein products (AOPP) on proliferation, differentiation, and oxidative stress in rat osteoblasts.
METHODSThe cell proliferation and differentiation of osteoblasts isolated from neonatal SD rat skull were evaluated following treatment with different concentrations (50, 100, 200, and 400 µg/ml) of AOPP using CCK-8 kit and ALP assay kit, respectively. The levels of reactive oxygen species (ROS) in the treated cells were analyzed using 2', 7'-dichlorofluorescin diacetate, and the transcription levels of ALP, collagen I and RAGE were assessed using real-time PCR.
RESULTSCompared with the control group, AOPP-treated osteoblasts showed obviously inhibited proliferation and differentiation with down-regulated expressions of ALP and collagen I and increased ROS production and RAGE expression.
CONCLUSIONAOPP can inhibit the proliferation and differentiation of rat osteoblasts partially by up-regulating RAGE and inducing ROS production.
