Effects of mesangial cell on proliferation and function of osteoblast in vitro.
- Author:
Feng WU
1
;
Ri-yang LIN
;
Li-qun HE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; Male; Mesangial Cells; physiology; Osteoblasts; physiology; Osteopontin; physiology; Rats; Rats, Sprague-Dawley; Transforming Growth Factor beta; physiology
- From: China Journal of Orthopaedics and Traumatology 2012;25(4):324-327
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects and mechanism of mesangial cell (MC) on proliferation and function of osteoblast in vitro from growth factors.
METHODSOsteoblast cultured in vitro were divided into normal blood serum group and MC group and cultured supernatant fluid by 10% serum culture medium and 20% MC culture medium. MTF was used to checked the proliferation of osteoblast at the end of 24, 48, 72, 120 h; Expression of BGP and OPN were tested 72 h and 144 h after culture; Expression of IGF-alpha and TGF-beta were tested 144 h after culture.
RESULTSThe proliferation of osteoblast in MC group was obviously higher than normal blood serum group in different times (P < 0.05); The level of BGP and OPN of osteoblast was obviously higher than normal blood serum group (11.3%, 16.4%, 55.0% and 39.6%); and the level of IGF-alpha and TGF-beta of osteoblast was obviously higher than normal blood serum group (10.1% and 47.7%).
CONCLUSIONMC can directly act on osteoblast, promote the proliferation and function of osteoblast by promoting secretion of TGF-beta.
