Effect of chemotherapy with cisplatin and rapamycin against Hep-2 cells in vitro.
- Author:
Wen-bin LEI
1
;
Tao JIA
;
Zhen-zhong SU
;
Wei-ping WEN
;
Xiao-lin ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Carcinoma, Squamous Cell; pathology; Cell Proliferation; drug effects; Cisplatin; pharmacology; Drug Synergism; Humans; Laryngeal Neoplasms; pathology; Sirolimus; pharmacology; Tumor Cells, Cultured
- From: Journal of Southern Medical University 2008;28(10):1838-1841
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of combined use of rapamycin and cisplatin in the chemotherapy of Hep-2 cells in vitro.
METHODSThe inhibitory effects of rapamycin and cisplatin, used alone or in combination, on the proliferation of Hep-2 cells were measured with MTT assay and median-effect plot analysis. The cell cycle changes after the treatment were analyzed using flow cytometry and Hoechst 33258 immunofluorescence staining.
RESULTSThe IC50 of rapamycin and cisplatin for inducing growth arrest of Hep-2 cells was 11.03 nmol/L and 8.81 micromol/L, respectively. Rapamycin alone caused cell cycle arrest of the Hep-2 cells in G1 phase. Rapamycin and cisplatin showed synergistic effects in the chemotherapy of Hep-2 cells (q > 1.15, King's Formula), causing significantly increased apoptosis ratio and growth inhibition rate of Hep-2 cells.
CONCLUSIONCombined use of rapamycin and cisplatin significantly improves the chemotherapeutic effect against Hep-2 cells.
