Genetic polymorphism analysis of 3 short tandem repeat loci on chromosome 21 in Chinese Han population in Shaanxi Province.
- Author:
Xiang WANG
1
;
Wei CHEN
;
Le ZHAO
;
Ping ZHOU
;
Xu LI
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; ethnology; genetics; Chromosomes, Human, Pair 21; genetics; Female; Genetic Markers; Heterozygote; Humans; Male; Microsatellite Repeats; genetics; Polymorphism, Genetic; genetics
- From: Journal of Southern Medical University 2008;28(10):1906-1908
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the polymorphism of 3 short tandem repeat (STR) loci D21S1442, D21S1413 and D21S1809 on human chromosome 21 in Shaanxi Han nationality in China.
METHODSBlood samples anti-coagulated with EDTA were collected from 160 unrelated Chinese Han individuals in Shaanxi Province. The genomic DNA was extracted with Chelex-100, amplified by PCR and analyzed using PAGE with silver staining.
RESULTSNine, eight and seven alleles were detected at the loci of D21S1442, D21S1413 and D21S1809, respectively. The genotype distributions of all the 3 loci were in accordance with Hardy-Weinberg equilibrium. The heterozygosities of the 3 loci were 0.750, 0.742 and 0.694, respectively. No linkage disequilibrium was found between the loci.
CONCLUSIONThe 3 loci on chromosome 21 have high heterozygosity and polymorphic information content in Shaanxi Han population, suggesting their value as potentially useful genetic markers.
