Angiotensin II type 2 receptors participate in the regulation of inflammatory cytokine secretion in adult rat hypertrophied cardiomyocytes.
- Author:
Juan ZHOU
1
;
Xin XU
;
Jin-Jun LIU
;
Yuan-Xi LIN
;
Guang-Dao GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Cytokines; secretion; Hypertrophy; metabolism; pathology; Inflammation Mediators; metabolism; Myocytes, Cardiac; metabolism; pathology; Rats; Rats, Sprague-Dawley; Receptor, Angiotensin, Type 2; metabolism
- From: Journal of Southern Medical University 2008;28(11):1971-1973
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of angiotensin II (AngII) type 2 (AT2) receptors on pressure overload-induced inflammatory cytokine secretion in adult rat hypertrophied cadiomyocytes.
METHODSRat models of left ventricular hypertrophy induced by pressure overload was established by placing a band around the abdominal aortic of the rats, from which the hypertrophied cadiomyocytes were isolated and purified 8 weeks later. The isolated cardiomyocytes were treated with AngII plus losartan or AngII plus PD123319, and 36 h after the treatments, the expression levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and IL-6 in the supernatant were detected using radioimmunoassay.
RESULTSAngII induced TNF-alpha and IL-1beta secretion from the hypertrophied cardiomyocyets, and pretreatment of the cells with PD123319, but not losartan, decreased their secretion. IL-6 level was not detected in the supernatant.
CONCLUSIONAngII-induced the expression of inflammatory cytokines in adult rat hypertrophied cardiomyocytes is mediated mainly by AT2, not by AT1 receptors.
