Isolation, culture and purification of olfactory ensheathing cells from human fetal olfactory mucosa.
- Author:
Qiang LI
1
;
Xi-Jing HE
;
Jian-Feng SHI
;
Bin WANG
;
Zhen-Zhong ZHU
;
Quan-Jin ZANG
;
Pei FAN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Culture Techniques; methods; Cell Separation; methods; Cells, Cultured; Culture Media; Fetus; Humans; Neurotrophin 3; pharmacology; Olfactory Bulb; cytology; Olfactory Mucosa; cytology
- From: Journal of Southern Medical University 2008;28(11):1974-1976
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the method for obtaining olfactory ensheathing cells from human fetal olfactory mucosa by cell culture for selective adhesion in the presence of neurotrophin-3 (NT3) and low-concentration serum.
METHODSThe olfactory ensheathing cells were cultured alternatively in DMEM/F12 culture medium containing 10% fetal bovine serum (FBS) and the medium containing NT3 and 2.5% FBS every 72 h. The cells were observed for morphological changes and identified using immunocytochemistry with P75NTR and GFAP, and the cell purity was estimated.
RESULTSThe olfactory ensheathing cells from human fetal olfactory mucosa were positive for P75(NTR) and GFAP, and in in vitro culture, the cells exhibited dipolar or tripolar appearance with long thin neurites. On the 9th day of cell culture, the purity of the olfactory ensheathing cells reached about 83%.
CONCLUSIONThe olfactory ensheathing cells can be obtained by in vitro culture for selective adhesion in the presence of NT3 and low-concentration serum.
