Small interfering RNA targeting C-erbB-2 gene increases the radiosensitivity of lung adenocarcinoma cells in vitro.
- Author:
Hua-Shan SHI
1
;
De-Lian REN
;
Xin-Mei CAO
;
Li HUANG
;
Yao JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; pathology; Apoptosis; drug effects; radiation effects; Cell Line, Tumor; Genes, erbB-2; genetics; Humans; Lung Neoplasms; genetics; pathology; RNA Interference; RNA, Small Interfering; genetics; Radiation Dosage; Radiation Tolerance; genetics
- From: Journal of Southern Medical University 2008;28(11):1977-1980
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of small interfering RNA (siRNA) targeting C-erbb-2 oncogene on the radiosensitivity of C-erbb-2-overexpressing lung adenocarcinoma cell line.
METHODSFour pairs of siRNA targeting the coding sequence of C-erbb-2 mRNA were synthesized and their interference effects were evaluated using quantitative real-time fluorescence PCR. The siRNA with the best interference effects was transfected into Calu-3 cells, which were then exposed to 2 or 5 Gy irradiation, with the cells with transfection or irradiation alone as the control groups. The cell apoptosis after the treatment was detected using annexin V-FITC Kit.
RESULTSThe apoptosis rate of the Calu cells was 7.767-/+0.551 in the blank group, 14.400-/+1.114 in the interference group, 11.867-/+0.737 in 2 Gy irradition group, 23.000-/+1.664 in 2 Gy irradiation + interference group, 16.100-/+0.624 in 5 Gy irradiation group, and 27.900-/+1.709 in 5 Gy irradiation+interference group.
CONCLUSIONThe siRNA targeting C-erbb-2 gene can enhance the radiosensitivity of Calu-3 cells to gamma-ray and increase their apoptosis rate following gamma-ray exposure in vitro.
